Rv2940c, (MTCY24G1.09, MTCY19H9.08c), len: 2111 aa. Probable mas, mycocerosic acid synthase membrane associated, multifunctional enzyme (see citations below),almost identical to Q02251|MCAS_MYCBO|mas mycocerosic acid synthase from Mycobacterium bovis (2110 aa), FASTA scores: opt: 13226, E(): 0, (95.8% identity in 2115 aa overlap) (see Mathur & Kolattukudy 1992); and equivalent to Q9CD78|mas|ML0139 putative mycocerosic synthase from Mycobacterium leprae (2116 aa), FASTA scores: opt: 12142,E(): 0, (87.95% identity in 2119 aa overlap); and Q49624|PKS3|MASA|ML1229|B1170_C2_209 probable myc [...]

Conserved protein; Catalyzes the sulfuryl group transfer from 3'- phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid and the most abundant sulfated metabolite found in Mycobacterium tuberculosis, that is a potential virulence factor thought to mediate host-pathogen interactions.

Probable polyketide synthase Pks5; Polyketide synthase likely involved in the biosynthesis of a polymethyl-branched fatty acid (PMB-FA) that might only be produced during host infection. Is required for the full virulence of M.tuberculosis during host infection.

Phenolpthiocerol synthesis type-I polyketide synthase PpsC; Involved in the elongation of either C22-24 fatty acids by the addition of malonyl-CoA and methylmalonyl-CoA extender units to yield phthiocerol derivatives.

Polyketide synthase Pks13; Rv3800c, (MTV026.05c), len: 1733 aa. Probable pks13,polyketide synthase, equivalent to Q9CDB1|PKS13|ML0101 polyketide synthase from Mycobacterium leprae (1784 aa),FASTA scores: opt: 7454, E(): 0, (83.6% identity in 1748 aa overlap); and similar to Q9Z5K6|ML2357|MLCB12.02c putative polyketide synthase from Mycobacterium leprae (1871 aa),FASTA scores: opt: 1682, E(): 1.2e-85, (38.3% identity in 1096 aa overlap). Also similar in part to many e.g. Q9ADL6|SORA soraphen polyketide synthase a from Polyangium cellulosum (6315 aa) FASTA scores: opt: 1422, E(): 1e-70,( [...]

Polyketide synthase Pks12; Rv2048c, (MTV018.35c), len: 4151 aa. Pks12,polyketide synthase similar to many. Contains 2x PS00012 Phosphopantetheine attachment site, 2x PS00606 Beta-ketoacyl synthases active site, and PS00343 Gram-positive cocci surface proteins 'anchoring' hexapeptide. Nucleotide position 2297976 in the genome sequence has been corrected, G:A resulting in S3004L.

Probable polyketide beta-ketoacyl synthase Pks4; Polyketide synthase involved in the biosynthesis of methyl- branched fatty acids such as mycolipanoic, mycolipenic (phthienoic) and mycolipodienoic acids required for the synthesis of a major class of polyacylated trehaloses. Catalyzes the elongation of CoA esters of long-chain fatty acids by incorporation of three methylmalonyl (but not malonyl) residues, to form trimethyl-branched fatty-acids.

Possible electron transfer protein FdxB; Rv3554, (MTCY06G11.01, MTCY03C7.01c), len: 685 aa. Possible fdxB, two-domain protein, with ferredoxin reductase electron transfer component in C-terminal part and unknown function in N-terminal part. Indeed, N-terminal end is similar to O85832 hypothetical 36.1 KDA protein from Sphingomonas aromaticivorans strain F199 (catabolic plasmid pNL1) (309 aa), FASTA scores: opt: 615, E(): 2.5e-30,(33.1% identity in 311 aa overlap); and P73428|SLL1468 hypothetical 36.2 KDA protein from Synechocystis sp. strain PCC 6803 (312 aa), FASTA scores: opt: 317, E [...]

Conserved integral membrane transport protein MmpL8; Required for the biosynthesis and the transport across the inner membrane of sulfolipid-1 (SL-1), which is a major cell wall lipid of pathogenic mycobacteria. Could also transport SL1278 (2-palmitoyl-3- (C43)-phthioceranyl-alpha, alpha'-D-trehalose-2'-sulfate), which is the precursor of SL-1. Required for virulence.