Preprotein translocase secY subunit (TC 3.A.5.1.1); The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.

LSU ribosomal protein L11p (L12e); Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.

Transcription antitermination protein NusG; Participates in transcription elongation, termination and antitermination.

Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1); Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane; Belongs to the SecA family.

Signal recognition particle, subunit Ffh SRP54 (TC 3.A.5.1.1); Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex in [...]

Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1).

LSU ribosomal protein L33p @ LSU ribosomal protein L33p, zinc-dependent; Belongs to the bacterial ribosomal protein bL33 family.

LSU ribosomal protein L1p (L10Ae); Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.

Preprotein translocase subunit YajC (TC 3.A.5.1.1); The SecYEG-SecDF-YajC-YidC holo-translocon (HTL) protein secretase/insertase is a supercomplex required for protein secretion, insertion of proteins into membranes, and assembly of membrane protein complexes. While the SecYEG complex is essential for assembly of a number of proteins and complexes, the SecDF-YajC-YidC subcomplex facilitates these functions.